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Switching from an esterase to a hydroxynitrile lyase mechanism requires only two amino acid substitutions.
Padhi, Santosh Kumar; Fujii, Ryota; Legatt, Graig A; Fossum, Sara L; Berchtold, Reto; Kazlauskas, Romas J.
Affiliation
  • Padhi SK; Department of Biochemistry, Molecular Biology, and Biophysics, and the Biotechnology Institute, University of Minnesota, 1479 Gortner Avenue, Saint Paul, MN 55108, USA.
Chem Biol ; 17(8): 863-71, 2010 Aug 27.
Article in En | MEDLINE | ID: mdl-20797615
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Amino Acid Substitution / Aldehyde-Lyases / Esterases Language: En Journal: Chem Biol Journal subject: BIOLOGIA / BIOQUIMICA / QUIMICA Year: 2010 Document type: Article Affiliation country: Estados Unidos Country of publication: Estados Unidos
Fulltext
Add to My VHL
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Amino Acid Substitution / Aldehyde-Lyases / Esterases Language: En Journal: Chem Biol Journal subject: BIOLOGIA / BIOQUIMICA / QUIMICA Year: 2010 Document type: Article Affiliation country: Estados Unidos Country of publication: Estados Unidos