Pioglitazone has direct effects on insulin sensitivity and intracellular lipid content in L6 skeletal muscle cells.
Horm Metab Res
; 42(13): 987-90, 2010 Dec.
Article
in En
| MEDLINE
| ID: mdl-20945270
ABSTRACT
Using L6 skeletal muscle cell line, rendered insulin resistant by incubation with triglyceride-rich lipoproteins (TGRLs), we sought to answer the question whether pioglitazone has direct effects on this cell line. Incubation of L6 cells with TGRLs led to an increase in the intramyocellular triglyceride content. Moreover, TGRLs led to a reduction in insulin-stimulated glycogen content and GSK-3 phosphorylation. All these changes induced by TGRLs could be antagonized by incubation of L6 cells with pioglitazone. The PPAR-γ antagonist GW9662 reversed the pioglitazone effects. We conclude that pioglitazone has direct insulin-sensitizing effects on the L6 skeletal muscle cell line, which are paralleled by a reduction in intramyocellular triglyceride accumulation.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Muscle, Skeletal
/
Muscle Cells
/
Intracellular Space
/
Thiazolidinediones
/
Lipid Metabolism
/
Insulin
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Horm Metab Res
Year:
2010
Document type:
Article
Affiliation country:
Austria