Effects of insulin-like growth factor-I and platelet-rich plasma on sciatic nerve crush injury in a rat model.
J Neurosurg
; 114(2): 522-8, 2011 Feb.
Article
in En
| MEDLINE
| ID: mdl-21029038
ABSTRACT
OBJECT Local administration of insulin-like growth factor-I (IGF-I) has been shown to increase the rate of axon regeneration in crush-injured and freeze-injured rat sciatic nerves. Local administration of platelet-rich plasma (PRP) has been also shown to have a measurable effect on facial nerve regeneration after transection in a rat model. The objective of the study was to compare the effects of locally administered IGF-I and PRP on the parameters of the Sciatic Function Index (SFI), sensory function (SF), axon count, and myelin thickness/axon diameter ratio (G-ratio) in a rat model of crush-injured sciatic nerves. METHODS:
The right sciatic nerve of Wistar albino rats (24 animals) was crushed using a Yasargil-Phynox aneurysm clip for 45 minutes. All animals were randomly divided into 3 groups Group 1 (control group) was treated with saline, Group 2 was treated with IGF-I, and Group 3 was treated with PRP. Injections were performed using the tissue expander's injection port with a connecting tube directed at the crush-injured site. Functional recovery was assessed with improvement in the SFI. Recovery of sensory function was using the pinch test. Histopathological examination was performed 3 months after the injury.RESULTS:
The SFI showed an improved functional recovery in the IGF-I-treated animals (Group 2) compared with the saline-treated animals (Group 1) 30 days after the injury. In IGF-I-treated rats, sensory function returned to the baseline level significantly faster than in saline-treated and PRP-treated rats as shown in values between SF-2 and SF-7. The G-ratios were found to be significantly higher in both experimental groups than in the control group.CONCLUSIONS:
This study suggests that the application of IGF-I to the crush-injured site may expedite the functional recovery of paralyzed muscle by increasing the rate of axon regeneration.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sciatic Nerve
/
Insulin-Like Growth Factor I
/
Sciatic Neuropathy
/
Platelet-Rich Plasma
/
Nerve Regeneration
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J Neurosurg
Year:
2011
Document type:
Article
Affiliation country:
Turquía