Tetraspanins CD9 and CD81 are molecular partners of trimeric FcÉRI on human antigen-presenting cells.
Allergy
; 66(5): 605-11, 2011 May.
Article
in En
| MEDLINE
| ID: mdl-21241315
ABSTRACT
BACKGROUND:
Most functions of tetraspanins are not related to cell-surface receptor ligand binding, but are mediated by direct interactions with their partner proteins. Functions of trimeric FcÉRI, expressed by antigen-presenting cells (APCs), range from amplification of allergic inflammatory reactions to their active suppression. Cell-type-specific protein-protein interactions might play a role in the regulation of these bidirectional tasks. Therefore, we intended to study the interactions of trimeric FcÉRI with tetraspanins.METHODS:
The expression levels of tetraspanins CD9, CD37, CD53, CD63, CD81, CD82, and CD151 on skin dendritic cells of atopic dermatitis (AD) patients or healthy individuals were detected by flow cytometry. Tetraspanin expression on FcÉRI(pos) and FcÉRI(neg) monocyte subpopulations was evaluated. Flow cytometry, confocal microscopy, immunoprecipitation, and immunoblotting experiments were performed to observe the relationship between tetraspanins CD9 and CD81 and FcÉRI. Furthermore, plate stimulation experiments were performed, and cytokines in the supernatants were detected.RESULTS:
We found that human FcÉRI(pos) APCs expressed high amounts of tetraspanins and that the tetraspanins CD9 and CD81 were associated with FcÉRI. Concomitant activation of FcÉRI and CD9 on human monocytes increased FcÉRI-mediated cytokine release.CONCLUSION:
Taken together, we show for the first time that CD9 and CD81 act as molecular partners of trimeric FcÉRI on human APC, which might be of importance in allergic diseases such as AD.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Dendritic Cells
/
Membrane Glycoproteins
/
Antigens, CD
/
Receptors, IgE
/
Dermatitis, Atopic
/
Antigen-Presenting Cells
Type of study:
Observational_studies
/
Risk_factors_studies
Limits:
Humans
Language:
En
Journal:
Allergy
Year:
2011
Document type:
Article
Affiliation country:
Alemania