Sensing scaffolds to monitor cellular activity using impedance measurements.
Biosens Bioelectron
; 26(7): 3303-8, 2011 Mar 15.
Article
in En
| MEDLINE
| ID: mdl-21256732
ABSTRACT
Scaffolds are cell adhesive matrices for the realisation of tissue constructs. Here we describe how scaffolds for tissue engineering can also be used as sensors for monitoring cellular activity such as adhesion and spreading. Carbon nanotube polymer composites were fabricated into membranes and scaffolds with electro-conductive properties. Impedance techniques were used to measure the effects of media and cell cultures on composite membranes and the results were analysed using lumped parameter models. We show that protein adhesion can be distinguished from cell adhesion as the impedance changes are much smaller for the latter (5%). In the presence of cells, impedance changes are of the order of 40% and can be correlated with adhesion, spreading and changes in cell density.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biosensing Techniques
/
Electric Impedance
/
Nanotubes, Carbon
/
Tissue Scaffolds
Limits:
Humans
Language:
En
Journal:
Biosens Bioelectron
Journal subject:
BIOTECNOLOGIA
Year:
2011
Document type:
Article
Affiliation country:
Italia