The effects and mechanism of xenogeneic adipocyte vaccine for the prevention of obesity in rats.
J Int Med Res
; 38(5): 1700-7, 2010.
Article
in En
| MEDLINE
| ID: mdl-21309484
ABSTRACT
The objectives of this study were to observe the effects of immunization with mouse mature adipocytes for the prevention of obesity in rats and to investigate their mechanism of action. Mouse mature adipocytes (3T3-L1) were injected as a vaccine into the abdominal cavity of rats. Control rats were injected with fibroblast cell lines (MRC-5 or NIH/3T3) or with 0.9% saline. Rats were fed a high calorie diet and body weight changes were used to evaluate obesity prevention. Immunohistochemical and immunofluorescence assays were used to investigate the mechanism of action. Results showed that obesity in rats can be prevented by immunization with xenogeneic mature mouse adipocytes. Body weight gain was inhibited in rats in the treatment group but not in the control groups and was statistically significant between the groups over the 19-week observation period. The assays demonstrated the presence of autoantibodies in rat adipocytes. It was concluded that vaccines of xenogeneic adipocytes can effectively prevent obesity in rats.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Immunotherapy, Active
/
Adipocytes
/
Obesity
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
En
Journal:
J Int Med Res
Year:
2010
Document type:
Article
Affiliation country:
China