A rapid latex agglutination assay for the determination of plasma fibrinogen.
Blood Coagul Fibrinolysis
; 1(4-5): 465-8, 1990 Oct.
Article
in En
| MEDLINE
| ID: mdl-2133224
ABSTRACT
A monoclonal antibody to intact fibrinogen has been employed to develop a rapid latex agglutination assay for the estimation of plasma fibrinogen. The monoclonal antibody, 45J, recognizes an epitope located in the mid-section of the carboxy terminal end of the A alpha-chain. The epitope is destroyed by plasmin digestion of fibrinogen and there is no immunoreactivity with soluble cross-linked fibrin degradation products. The latex agglutination assay developed with the antibody is unaffected by aprotinin or anticoagulants, such as citrate, heparin or EDTA. When this method was compared with functional clotting assays, excellent correlation was observed with normal and pathological samples. After sample collection and dilution, the assay takes just two minutes to complete. Therefore, this procedure offers a simple and rapid assay for the measurement of intact fibrinogen in plasma.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Fibrinogen
/
Latex Fixation Tests
/
Antibodies, Monoclonal
Limits:
Animals
/
Humans
Language:
En
Journal:
Blood Coagul Fibrinolysis
Journal subject:
ANGIOLOGIA
/
HEMATOLOGIA
Year:
1990
Document type:
Article