Antimicrobial activity and chemical composition of the essential oil of Hofmeisteria schaffneri.
J Pharm Pharmacol
; 63(4): 579-86, 2011 Apr.
Article
in En
| MEDLINE
| ID: mdl-21401611
ABSTRACT
OBJECTIVES:
The aims of this study were to establish the antimicrobial potential of Hofmeisteria schaffneri essential oil and its chemical composition.METHODS:
The essential oils of Hofmeisteria schaffneri harvested at flowering (batches I and IV) and non-flowering (batches II and III) seasons were prepared by hydrodistillation and analysed by GC and GC-MS. The aqueous and organic (CH(2) Cl(2) -MeOH 1 1) extracts were prepared by using infusion and maceration techniques, respectively. The in-vitro antimicrobial activity of the preparations and compounds against Candida albicans and some bacteria (Gram-negative and Gram-positive) was assessed using the broth dilution method in 96-microplate wells. KEYFINDINGS:
Forty-four compounds, representing â¼90% of the total constituents, were identified in the essential oil of Hofmeisteria schaffneri collected in flowering (batches I and IV) and non-flowering (batches II and III) seasons. In all cases, several thymol analogues were the major components of the oils (â¼65%); some small differences in the relative proportions of these constituents were observed. The infusion exhibited an antibacterial activity against Staphylococcus aureus and Bacillus subtilis, with a MIC value of 64 µg/ml in each case. The essential oil batches were active against Staphylococcus aureus, with MIC ranging from 48 to 192 µg/ml. They were, however, inactive against Gram-negative bacteria, including Pseudomonas aeruginosa, Escherichia coli and Salmonella typhi (MIC > 1024 µg/ml). On the other hand, the infusion of the plant as well as the oil from batch I displayed anti-Candida albicans activity, with MIC of 128 and 192 µg/ml, respectively. Finally, the organic extract did not displayed significant activity against the tested microorganisms (MIC ≥ 1024 µg/ml). Some of the compounds isolated from the plant were also tested. Compounds 8 and 38, which were present in the essential oils, displayed the best antibacterial effect against Gram-positive bacteria (MIC ranging between 32 and 64 µg/ml). Compounds 6 (present in the infusion) and 10 (present in all preparations) showed higher activity against the yeast (MIC = 128 µg/ml) than the remaining compounds, with MIC values ranging from 256 to 512 µg/ml.CONCLUSIONS:
The composition and antimicrobial activity of the oils changed slightly from flowering to non-flowering seasons. The results of the present investigation provide in-vitro scientific support for the use of the plant against skin infections in Mexican folk medicine.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oils, Volatile
/
Asteraceae
/
Anti-Infective Agents
Type of study:
Prognostic_studies
Language:
En
Journal:
J Pharm Pharmacol
Year:
2011
Document type:
Article