The 49 K subunit of NADH: ubiquinone reductase (complex I) from Neurospora crassa mitochondria: primary structure of the gene and the protein.
Curr Genet
; 18(1): 59-64, 1990 Jul.
Article
in En
| MEDLINE
| ID: mdl-2147127
ABSTRACT
The primary structure of the 49 K subunit of the respiratory chain NADHubiquinone reductase (complex I) from Neurospora crassa was determined by sequencing cDNA, genomic DNA and the N-terminus of the mature protein. The sequence lengths correlate to a molecular mass of 54,002 daltons for the preprotein and 49,239 daltons for the mature protein. The presequence consists of 42 amino acids of typical composition for sequences which target nuclear-encoded proteins into mitochondria. The mature protein consists of 436 amino acids and shows 64% similarity to a 49 K subunit of bovine heart NADHubiquinone reductase and 33% to a predicted translation product of an open reading frame in the chloroplast DNAs of Marchantia polymorpha and Nicotiana tabacum. Evidence for an iron-sulfur cluster in the subunit is discussed.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Quinone Reductases
/
Neurospora crassa
Language:
En
Journal:
Curr Genet
Year:
1990
Document type:
Article