Transfection efficiency for size-separated cells synchronized in cell cycle by microfluidic device.
Biomed Microdevices
; 13(4): 725-9, 2011 Aug.
Article
in En
| MEDLINE
| ID: mdl-21479536
ABSTRACT
Non-viral system generally demonstrates less efficacious in transgene delivery than viral system; however it represents a safer alternative to viral system. In this study, transfection efficiency for human hepatocellular liver carcinoma cells synchronized in cell cycle at G0/G1 phase, which was sorted in size with a microfluidic device based on hydrodynamic filtration, was investigated by using a reverse transfection method. The synchronized cells were recovered at the yield of 80% from the micro-channel, and green fluorescent protein gene encoding plasmid mixed with lipofectoamine was transfected. The transfection efficiency of the cells at G0/G1 phase was 1.8 times higher than non-synchronized cells. The manipulation of cell cycle status could increase transfection efficiency in non-viral system, indicating size-based cell cycle synchronization is a powerful tool as a noninvasive method for bioscience and biotechnology.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transfection
/
Resting Phase, Cell Cycle
/
G1 Phase
/
Microfluidic Analytical Techniques
Limits:
Humans
Language:
En
Journal:
Biomed Microdevices
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2011
Document type:
Article
Affiliation country:
Japón