Ultrasound-mediated intracellular drug delivery using microbubbles and temperature-sensitive liposomes.
J Control Release
; 155(3): 442-8, 2011 Nov 07.
Article
in En
| MEDLINE
| ID: mdl-21689699
ABSTRACT
A novel two-step protocol for intracellular drug delivery has been evaluated in vitro. As a first step TO-PRO-3 (a cell-impermeable dye that displays a strong fluorescence enhancement upon binding to nucleic acids) encapsulated in thermosensitive liposomes was released after heating to 42°C. A second step consisted of ultrasound-mediated local permeabilization of cell membrane allowing TO-PRO-3 internalization observable as nuclear staining. Only the combination of two consecutive steps - heating and sonication in the presence of SonoVue microbubbles led to the model drug TO-PRO-3 release from the thermosensitive liposomes and its intracellular uptake. This protocol is potentially beneficial for the intracellular delivery of cell impermeable drugs that suffer from rapid clearance and/or degradation in blood and are not intrinsically taken up by cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carbocyanines
/
Drug Delivery Systems
/
High-Energy Shock Waves
/
Microbubbles
/
Hot Temperature
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
J Control Release
Journal subject:
FARMACOLOGIA
Year:
2011
Document type:
Article
Affiliation country:
Francia