Functional molecular morphology of anterior pituitary cells, from hormone production to intracellular transport and secretion.
Med Mol Morphol
; 44(2): 63-70, 2011 Jun.
Article
in En
| MEDLINE
| ID: mdl-21717308
ABSTRACT
Combined in situ hybridization (ISH) and immunohistochemistry (IHC) under electron microscopy (EM-ISH & IHC) has sufficient ultrastructural resolution to provide two-dimensional images of subcellular localization of pituitary hormone and its mRNA in a pituitary cell. The advantages of semiconductor nanocrystals (Quantum dots; Qdots) and confocal laser scanning microscopy (CLSM) enable us to obtain three-dimensional images of the subcellular localization of pituitary hormone and its mRNA. Both EM-ISH & IHC and ISH & IHC using Qdots and CLSM are useful for understanding the relationship between protein and mRNA simultaneously in two or three dimensions. CLSM observation of rab3B and SNARE proteins such as SNAP-25 and syntaxin revealed that both rab3B and SNARE system proteins play an important role and work together as the exocytotic machinery in anterior pituitary cells. Another important issue is the intracellular transport and secretion of pituitary hormone. An experimental pituitary cell line, the GH3 cell, in which growth hormone (GH) is linked to enhanced yellow fluorescein protein (EYFP), has been developed. This stable GH3 cell secretes GH linked to EYFP upon being stimulated by Ca(2+) influx or Ca(2+) release from storage. This GH3 cell is useful for real-time visualization of the intracellular transport and secretion of GH. These three methods enable us to visualize consecutively the processes of transcription, translation, transport, and secretion of pituitary hormone.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA, Messenger
/
Growth Hormone
/
Rab3 GTP-Binding Proteins
/
Imaging, Three-Dimensional
/
Qa-SNARE Proteins
/
Synaptosomal-Associated Protein 25
Limits:
Animals
/
Humans
Language:
En
Journal:
Med Mol Morphol
Journal subject:
BIOLOGIA MOLECULAR
/
PATOLOGIA
Year:
2011
Document type:
Article
Affiliation country:
Japón