Pros and cons of benzodiazepines screening in human saliva by ion mobility spectrometry.
Anal Bioanal Chem
; 401(6): 1935-48, 2011 Oct.
Article
in En
| MEDLINE
| ID: mdl-21805317
ABSTRACT
The usefulness of ion mobility spectrometry as a screening methodology for the on-site benzodiazepine analysis in saliva samples has been critically evaluated. The procedure involved the injection of clear supernatant extracts after centrifugation and provided limit of detection values ranging from 2.0 to 18 µg L(-1), and a precision, expressed as relative standard deviation, from 2.9% to 16%, depending on the different benzodiazepines studied. Those values are appropriate for their positive identification in saliva samples in which benzodiazepine concentration, after a chronic or acute dose, is in the range of 2-30 µg L(-1). Problems related with overlapped benzodiazepine signals have been successfully overcome by application of multivariate curve resolution, which is a helpful tool to improve the resolution of the technique, without sacrificing the method simplicity and frequency of analysis. The possibility of false positives caused by the presence of interferents with the same drift time as the benzodiazepines and the possibility of false negatives due to the presence of interferents by competitive ionization have been critically evaluated. The satisfactory results obtained for the analysis of real saliva samples after an acute dose of diazepam through sublingual and oral intakes confirm the capability of the technique to be used as a screening methodology in the analysis of benzodiazepines in oral fluids.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Saliva
/
Mass Spectrometry
/
Benzodiazepines
/
Hypnotics and Sedatives
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Screening_studies
Limits:
Adult
/
Humans
Language:
En
Journal:
Anal Bioanal Chem
Year:
2011
Document type:
Article
Affiliation country:
España