Absence of altered autophagy after myocardial ischemia in diabetic compared with nondiabetic mice.
Coron Artery Dis
; 22(7): 479-83, 2011 Nov.
Article
in En
| MEDLINE
| ID: mdl-21811153
ABSTRACT
OBJECTIVES:
The extent of autophagy in myocardium following persistent ischemia and the effects of insulin resistance and diabetes on cardiac autophagy following myocardial infarction (MI) have not been well elucidated. It is generally thought that autophagy reflects the nutritional status of cells, presumably alterable by diabetes. It has been conjectured that diminution of autophagy early after the onset of MI may preserve jeopardized myocardium thereby improving prognosis.METHODS:
Ten-week-old nondiabetic C57BL6 mice, 20-week-old diabetic and nondiabetic C57BL6 mice were subjected to MI for 4 weeks. Hearts from these mice were harvested and assayed for markers of autophagy.RESULT:
Hearts of 10-week-old C57BL6 mice subjected to 4 weeks of MI had similar levels of LC3-II, a protein indicator of autophagy, as measured by western blotting compared with hearts from sham operated controls. In 20-week-old C57BL6 mice rendered diabetic by feeding a high-fat diet, the amounts of autophagy were comparable to those in 20-week-old nondiabetic C57BL6 mice on a normal diet.CONCLUSION:
The magnitude of autophagy in the heart after infarction is of very modest extent and is not modulated by diabetes. Thus, diminution of autophagy is not likely to reduce infarct size or attenuate late negative remodeling after MI in patients with diabetes.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Autophagy
/
Myocardial Ischemia
/
Diabetes Complications
/
Diabetes Mellitus, Experimental
/
Myocardial Infarction
/
Myocardium
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Coron Artery Dis
Journal subject:
ANGIOLOGIA
/
CARDIOLOGIA
Year:
2011
Document type:
Article
Affiliation country:
Estados Unidos