Hepatocytes produce tumor necrosis factor-α and interleukin-6 in response to Porphyromonas gingivalis.
J Periodontal Res
; 47(1): 89-94, 2012 Feb.
Article
in En
| MEDLINE
| ID: mdl-21895661
ABSTRACT
BACKGROUND AND OBJECTIVE:
The liver plays a major role in clearing systemic bacterial infections. In addition, inflammatory cytokines produced in the liver play a critical role in systemic cytokine levels. The aim of this study was to investigate the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) by hepatocytes in response to periodontal pathogens. MATERIAL ANDMETHODS:
The mouse hepatic carcinoma cell line Hepa-1.6 and the mouse macrophage-like cell line RAW 264 were co-cultured in Transwell insert plates. Cells were stimulated with bacterial extracts prepared from Porphyromonas gingivalis and the induction of TNF-α and IL-6 was measured using real-time PCR and ELISA.RESULTS:
After stimulation with bacteria, the induction of TNF-α and IL-6 was observed in RAW 264 cells and Hepa-1.6 cells. Significant reduction of TNF-α mRNA expression in Hepa-1.6 cells was observed after treatment with antibody to TNF-α.CONCLUSION:
The results obtained in the present study show that P. gingivalis extract induces TNF-α and IL-6 in an in vitro liver model and that macrophage-derived TNF-α mediates the induction of TNF-α in hepatocytes.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Interleukin-6
/
Tumor Necrosis Factor-alpha
/
Porphyromonas gingivalis
/
Hepatocytes
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J Periodontal Res
Year:
2012
Document type:
Article
Affiliation country:
Japón