Vitamin E deficiency decreases long-chain PUFA in zebrafish (Danio rerio).
J Nutr
; 141(12): 2113-8, 2011 Dec.
Article
in En
| MEDLINE
| ID: mdl-22013196
ABSTRACT
α-Tocopherol is a required, lipid-soluble antioxidant that protects PUFA. We hypothesized that α-tocopherol deficiency in zebrafish compromises PUFA status. Zebrafish were fed for 1 y either an α-tocopherol-sufficient (E+; 500 mg α-tocopherol/kg) or -deficient (E-; 1.1 mg α-tocopherol/kg) diet containing α-linolenic (ALA) and linoleic (LA) acids but without arachidonic acid (ARA), EPA, or DHA. Vitamin E deficiency in zebrafish decreased by ~20% (n-6) (P < 0.05) and (n-3) (P < 0.05) PUFA and increased the (n-6)(n-3) PUFA ratio (P < 0.05). In E- compared to E+ females, long chain-PUFA status was impaired, as assessed by a ~60% lower DHAALA ratio (P < 0.05) and a ~50% lower ARALA ratio (P < 0.05). fads2 (P < 0.05) and elovl2 (P < 0.05) mRNA expression was doubled in E- compared to E+ fish. Thus, inadequate vitamin E status led to a depletion of PUFA that may be a result of either or both increased lipid peroxidation and an impaired ability to synthesize sufficient PUFA, especially (n-3) PUFA.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vitamin E Deficiency
/
Diet
/
Fatty Acids, Unsaturated
Limits:
Animals
Language:
En
Journal:
J Nutr
Year:
2011
Document type:
Article
Affiliation country:
Estados Unidos