CpG site-specific hypermethylation of p16INK4α in peripheral blood lymphocytes of PAH-exposed workers.
Cancer Epidemiol Biomarkers Prev
; 21(1): 182-90, 2012 Jan.
Article
in En
| MEDLINE
| ID: mdl-22028397
ABSTRACT
BACKGROUND:
Sufficient epidemiologic evidence shows an etiologic link between polycyclic aromatic hydrocarbons (PAH) exposure and lung cancer risk. While the genetic modifications have been found in PAH-exposed population, it is unclear whether gene-specific methylation involves in the process of PAH-associated biologic consequence.METHODS:
Sixty-nine PAH-exposed workers and 59 control subjects were recruited. Using bisulfite sequencing, we examined the methylation status of p16(INK4α) promoter in peripheral blood lymphocytes (PBL) from PAH-exposed workers and in benzo(a)pyrene (BaP)-transformed human bronchial epithelial (HBE) cells. The relationships between p16(INK4α) methylation and the level of urinary 1-hydroxypyrene (1-OHP) or the frequency of cytokinesis block micronucleus (CBMN) were analyzed.RESULTS:
Compared with the control group, PAH-exposed workers exhibited higher levels of urinary 1-OHP (10.62 vs. 2.52 µg/L), p16(INK4α) methylation (7.95% vs. 1.14% for 22 "hot" CpG sites), and CBMN (7.28% vs. 2.92%) in PBLs. p16(INK4α) hypermethylation in PAH-exposed workers exhibited CpG site specificity. Among the 35 CpG sites we analyzed, 22 were significantly hypermethylated. These 22 hypermethylated CpG sites were positively correlated to levels of urinary 1-OHP and CBMN in PBLs. Moreover, the hypermethylation and suppression of p16 expression was also found in BaP-transformed HBER cells.CONCLUSION:
PAH exposure induced CpG site-specific hypermethylation of p16(INK4α) gene. The degree of p16(INK4α) methylation was associated with the levels of DNA damage and internal exposure. IMPACT p16(INK4α) hypermethylation might be an essential biomarker for the exposure to PAHs and for early diagnosis of cancer.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polycyclic Aromatic Hydrocarbons
/
Lymphocytes
/
DNA Methylation
/
Genes, p16
/
Cyclin-Dependent Kinase Inhibitor p16
Type of study:
Etiology_studies
/
Risk_factors_studies
/
Screening_studies
Limits:
Adult
/
Humans
Language:
En
Journal:
Cancer Epidemiol Biomarkers Prev
Journal subject:
BIOQUIMICA
/
EPIDEMIOLOGIA
/
NEOPLASIAS
Year:
2012
Document type:
Article
Affiliation country:
China