Culture-independent approaches for studying viruses from hypersaline environments.
Appl Environ Microbiol
; 78(6): 1635-43, 2012 Mar.
Article
in En
| MEDLINE
| ID: mdl-22247131
ABSTRACT
Hypersaline close-to-saturation environments harbor an extremely high concentration of virus-like particles, but the number of haloviruses isolated so far is still very low. Haloviruses can be directly studied from natural samples by using different culture-independent techniques that include transmission electron microscopy, pulsed-field gel electrophoresis, and different metagenomic approaches. Here, we review the findings of these studies, with a main focus on the metagenomic approaches. The analysis of bulk viral nucleic acids directly retrieved from the environment allows estimations of viral diversity, activity, and dynamics and tentative host assignment. Results point to a diverse and active viral community in constant interplay with its hosts and to a "hypersalineness" quality common to viral assemblages present in hypersaline environments that are thousands of kilometers away from each other.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Virology
/
Viruses
/
Environmental Microbiology
Language:
En
Journal:
Appl Environ Microbiol
Year:
2012
Document type:
Article
Affiliation country:
España