[Genetic manipulation and studying of differentiation properties of rat induced pluripotent stem cells].
Tsitologiia
; 53(12): 946-51, 2011.
Article
in Ru
| MEDLINE
| ID: mdl-22359953
ABSTRACT
Induced pluripotent stem (iPS) cells are derived from somatic cells reprogrammed to the pluripotent state by the induced expression of defined transcription factors, achieved for the first time by the seminal work of Takahashi and Yamanaka. This new type of pluripotent cells has offered new exciting options in regenerative medicine allowing the replacement of cells and organs with the patient's own cells thereby avoiding immunological complications. In order to develop such technologies in approved animal models, iPS cells were also generated from rodents. Of course, the most important model for studying of different diseases is rat. In this study, we present a method suitable for rat iPS cells genetic modification by stable transfection and show necessary conditions for the first stages of direct differentiation.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Differentiation
/
Induced Pluripotent Stem Cells
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Ru
Journal:
Tsitologiia
Year:
2011
Document type:
Article