A chelating derivative of alpha-melanocyte stimulating hormone as a potential imaging agent for malignant melanoma.
Br J Cancer
; 62(6): 919-22, 1990 Dec.
Article
in En
| MEDLINE
| ID: mdl-2257220
ABSTRACT
A chelating derivative of alpha-melanocyte stimulating hormone (MSH) has been synthesised, in which two molecules of the hormone are cross-linked by diethylenetriamine pentaacetic acid (DTPA). This compound, bisMSH-DTPA, was equipotent with MSH in an in vitro tyrosinase assay with Cloudman S91 melanoma cells. When DBA/2 mice bearing the same tumour were injected with bisMSH-DTPA labelled with the gamma-emitting isotope indium-111 (111In), the radioactivity became rapidly associated with the melanoma tissue. By 24 h post-injection, radioactivity in tumour tissue was significantly higher (P less than 0.001) than in spleen, lung, brain, eye and skin. Uptake of radioactivity by the tumours was inhibited by a 200-fold molar excess of MSH, whereas uptake by liver, kidney, spleen, lung, brain, eye and skin was unaffected. We conclude that bisMSH-DTPA may offer an alternative to antibody targeting in the imaging of malignant melanoma.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Indium Radioisotopes
/
Melanocyte-Stimulating Hormones
/
Pentetic Acid
/
Melanoma
Limits:
Animals
Language:
En
Journal:
Br J Cancer
Year:
1990
Document type:
Article
Affiliation country:
Reino Unido