Electron microscopy of biotinylated protein complexes bound to streptavidin monolayer crystals.
J Struct Biol
; 180(1): 249-53, 2012 Oct.
Article
in En
| MEDLINE
| ID: mdl-22584152
ABSTRACT
Chemical biotinylation of protein complexes followed by binding to two-dimensional (monolayer) crystals of streptavidin is shown to be an effective way to prepare cryo-EM specimens from samples at low protein concentration. Three different multiprotein complexes are used to demonstrate the generality of this method. In addition, native thermosomes, purified from Sulfolobus solfataricus P2, are used to demonstrate that a uniform distribution of Euler angles is produced, even though this particle is known to adopt a preferred orientation when other methods of cryo-EM specimen preparation are used.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biotin
/
Streptavidin
/
Cryoelectron Microscopy
Limits:
Animals
Language:
En
Journal:
J Struct Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2012
Document type:
Article
Affiliation country:
Estados Unidos