Gene knockout and knockin by zinc-finger nucleases: current status and perspectives.
Cell Mol Life Sci
; 70(16): 2969-83, 2013 Aug.
Article
in En
| MEDLINE
| ID: mdl-23161061
ABSTRACT
Zinc-finger nucleases (ZFNs) are engineered site-specific DNA cleavage enzymes that may be designed to recognize long target sites and thus cut DNA with high specificity. ZFNs mediate permanent and targeted genetic alteration via induction of a double-strand break at a specific genomic site. Compared to conventional homology-based gene targeting, ZFNs can increase the targeting rate by up to 100,000-fold; gene disruption via mutagenic DNA repair is similarly efficient. The utility of ZFNs has been shown in many organisms, including insects, amphibians, plants, nematodes, and several mammals, including humans. This broad range of tractable species renders ZFNs a useful tool for improving the understanding of complex physiological systems, to produce transgenic animals, cell lines, and plants, and to treat human disease.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Zinc Fingers
/
Endonucleases
/
Gene Knock-In Techniques
/
Gene Knockout Techniques
Limits:
Animals
/
Humans
Language:
En
Journal:
Cell Mol Life Sci
Journal subject:
BIOLOGIA MOLECULAR
Year:
2013
Document type:
Article
Affiliation country:
Alemania