Key role of estrogens and endothelial estrogen receptor α in blood flow-mediated remodeling of resistance arteries.
Arterioscler Thromb Vasc Biol
; 33(3): 605-11, 2013 Mar.
Article
in En
| MEDLINE
| ID: mdl-23288162
ABSTRACT
OBJECTIVE:
Flow- (shear stress-)mediated outward remodeling of resistance arteries is involved in collateral growth during postischemic revascularization. As this remodeling is especially important during pregnancy, we hypothesized that estrogens may be involved. A surgical model eliciting a local increase in blood flow in 1 mesenteric resistance artery was used in 3-month-old ovariectomized female rats either treated with 17-ß-estradiol (E2) or left untreated. METHODS ANDRESULTS:
After 14 days, arterial diameter was greater in high-flow arteries than in normal-flow vessels. An ovariectomy suppressed high-flow remodeling, while E2 restored it. High-flow remodeling was absent in mice lacking the estrogen receptor α but not estrogen receptor ß. The kinetics of inflammatory marker expression, macrophage infiltration, oxidative stress, and metaloproteinases expression were not altered by the absence of E2 after 2 and 4 days, that is, during remodeling. Nevertheless, E2 was required for the increase in endothelial nitric oxide synthase expression and activation at day 4 when diameter expansion occurs. Finally, the impact of E2 on the endothelium appeared crucial for high-flow remodeling, as this E2 action was abrogated in mice lacking endothelial NOS, as well as in Tie2-Cre(+) ERα(f/f) mice.CONCLUSIONS:
We demonstrate the essential role of E2 and endothelial estrogen receptor α in flow-mediated remodeling of resistance arteries in vivo.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vascular Resistance
/
Estrogen Replacement Therapy
/
Endothelial Cells
/
Estrogen Receptor alpha
/
Estradiol
/
Mesenteric Arteries
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Arterioscler Thromb Vasc Biol
Journal subject:
ANGIOLOGIA
Year:
2013
Document type:
Article
Affiliation country:
Francia