Endothelial cells actively concentrate insulin during its transendothelial transport.
Microcirculation
; 20(5): 434-9, 2013 Jul.
Article
in En
| MEDLINE
| ID: mdl-23350546
ABSTRACT
OBJECTIVE:
We examined insulins uptake and transendothelial transport by endothelial cells in order to (i) ascertain whether insulin accumulates within the cells to concentrations greater than in the media; (ii) compare trans endothelial insulin transport to that of inulin (using the latter as a tracer for passive transport or leaked); and; (iii) determine whether insulins transported depended on insulin action.METHODS:
Using 125I-insulin at physiologic concentrations we measured both the uptake and trans endothelial transport of insulin by bovine aortic endothelial cells and measured cell volume using tritiated 3-O-methylglucose.RESULTS:
Bovine aortic endothelial cells accumulate insulin to > five-fold above the media concentrations and the trans endothelial transport of insulin, but not inulin, is saturable and requires intact PI-3-kinase and MEK signaling.CONCLUSION:
The insulin receptor and downstream signaling from the receptor regulates endothelial insulin transport. Insulin is accumulated against a concentration gradient by the endothelial cell. We suggest that insulin uptake is rate limiting for insulin trans endothelial transport.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Endothelial Cells
/
Insulin
Limits:
Animals
Language:
En
Journal:
Microcirculation
Journal subject:
ANGIOLOGIA
Year:
2013
Document type:
Article
Affiliation country:
Estados Unidos