Using FRET to monitor protein-induced DNA bending: the TBP-TATA complex as a model system.
Methods Mol Biol
; 977: 203-15, 2013.
Article
in En
| MEDLINE
| ID: mdl-23436364
ABSTRACT
Proteins that bind to DNA can elicit changes in DNA conformation, such as bending and looping, which are important signals for later events such as transcription. TATA-binding protein (TBP) is one example of a protein that elicits a conformational change in DNA; TBP binds and sharply bends its recognition sequence, which is thought to facilitate the recruitment of other protein factors. Here we describe the use of fluorescence resonance energy transfer (FRET) to evaluate DNA bending using TBP as a model system. FRET is a useful technique to measure changes in DNA conformation due to protein binding because small changes in the distance between two fluorophores (2-10 nm) translate into large changes in energy transfer.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA
/
TATA Box
/
TATA-Box Binding Protein
/
Fluorescence Resonance Energy Transfer
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2013
Document type:
Article
Affiliation country:
Estados Unidos