[Alpha-adrenergic regulation of two calcium signal pathways in adipocytes].
Ross Fiziol Zh Im I M Sechenova
; 98(12): 1567-77, 2012 Dec.
Article
in Ru
| MEDLINE
| ID: mdl-23461200
ABSTRACT
Using selective receptor's agonist and antagonists we show that mouse white fat cells express alpha1A-, alpha2-adrenergic receptors, which activation with noradrenaline is capable of causing calcium responses different by formation mechanism. Adipocyte's calcium responses to alpha1-adrenoreceptor agonists are caused by alpha1A-type adrenoreceptor and suppressed by inhibitors of PLC-dependent pathway. Calcium responses to alpha2-adrenoreceptors agonists are realized only in the presence of more than 200 microM of L-arginine and suppressed by inhibitors of NOS-PKG-RyR pathway. The incubation of cells with L-arginine creates conditions for switching on the signal pathway with participation of eNOS --> NO --> sGC --> cGMP --> PKG --> CD38 --> RyR --> Ca2+ and for switching of the PLC - IP3R-dependent pathway. Adipocyte's calcium response to L-arginine represents a sharp impulse of the big amplitude and is mediated by alpha2-adrenoreceptors. L-arginine activating alpha2-adrenoreceptors and being the substrate of eNOS, realizes two functions in this pathway.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Norepinephrine
/
Calcium
/
Receptors, Adrenergic, alpha-2
/
Adipocytes
/
Adrenergic Antagonists
/
Adrenergic Agonists
/
Calcium Signaling
Limits:
Animals
Language:
Ru
Journal:
Ross Fiziol Zh Im I M Sechenova
Journal subject:
FISIOLOGIA
Year:
2012
Document type:
Article