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Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis.
Damron, F Heath; McKenney, Elizabeth S; Barbier, Mariette; Liechti, George W; Schweizer, Herbert P; Goldberg, Joanna B.
Affiliation
  • Damron FH; Department of Microbiology, Immunology, and Cancer Biology, University of Virginia Health System, Charlottesville, VA, USA.
Appl Environ Microbiol ; 79(13): 4149-53, 2013 Jul.
Article in En | MEDLINE | ID: mdl-23584769
We describe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Genes, Reporter / Gene Expression Profiling / Luciferases, Bacterial / Genetic Vectors / Gram-Negative Bacteria Type of study: Diagnostic_studies Language: En Journal: Appl Environ Microbiol Year: 2013 Document type: Article Affiliation country: Estados Unidos Country of publication: Estados Unidos
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Genes, Reporter / Gene Expression Profiling / Luciferases, Bacterial / Genetic Vectors / Gram-Negative Bacteria Type of study: Diagnostic_studies Language: En Journal: Appl Environ Microbiol Year: 2013 Document type: Article Affiliation country: Estados Unidos Country of publication: Estados Unidos