Hansschlegelia beijingensis sp. nov., an aerobic, pink-pigmented, facultatively methylotrophic bacterium isolated from watermelon rhizosphere soil.
Int J Syst Evol Microbiol
; 63(Pt 10): 3715-3719, 2013 Oct.
Article
in En
| MEDLINE
| ID: mdl-23625265
ABSTRACT
A novel Gram-stain-negative, aerobic, rod-shaped strain designated PG04(T) was isolated from the rhizosphere of watermelon plants cultivated in Beijing, China. A polyphasic taxonomic study was performed on the new isolate. On the basis of 16S rRNA gene sequence similarity studies, isolate PG04(T) belonged clearly to the genus Hansschlegelia and was most closely related to Hansschlegelia zhihuaiae (97.3â% similarity to the type strain). The predominant respiratory quinone was ubiquinone 10 (Q-10) and the polar lipid profile was composed of the major lipids diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The major fatty acids were C18ââ1ω7c (41.3â%), C19ââ0 cyclo ω8c (30.6â%) and C16ââ0 (19.1â%). The G+C content of the DNA was about 64.4 mol%. DNA-DNA hybridization experiments showed 34.4â% relatedness between strain PG04(T) and H. zhihuaiae DSM 18984(T). The results of physiological and biochemical tests and differences in fatty acid profiles allowed clear phenotypic differentiation of strain PG04(T) from the most closely related species in the genus, H. zhihuaiae. Strain PG04(T) therefore represents a novel species within the genus Hansschlegelia, for which the name Hansschlegelia beijingensis sp. nov. is proposed, with the type strain PG04(T) (â=âDSM 25481(T)â=âACCC 05759(T)).
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phylogeny
/
Soil Microbiology
/
Citrullus
/
Methylocystaceae
/
Rhizosphere
Country/Region as subject:
Asia
Language:
En
Journal:
Int J Syst Evol Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2013
Document type:
Article