[Plasma proteomic research on obesity subtype and non-obesity subtype of T2DM].

ABSTRACT

OBJECTIVE: With the increasingly serious epidemic situation of diabetes, plasma proteomic method and OFFGEL electrophoresis have been applied for screening different proteins between obese and non-obese T2DM patients, which may be used to further explain the mechanism of T2DM. METHODS: Twenty male T2DM volunteers (Obesity Subtype 10; Non-obesity Subtype 10) with the age of 18-44 years have been selected. The control group has been matched considering the factors of age, gender, etc. Albumin and IgG were removed from the plasma samples with highly specific immune-affinity method. Then the peptide-mixed samples were separated by pI with OFFGEL electrophoresis after solution digestion. Further separation and identification were performed by Nano HPLC-Chip-MS/MS system. Comparing the three groups, the differences were obtained and annotated on functions and its mechanism. RESULTS: 391, 415 and 371 proteins have been identified in the experimental groups and control group, respectively. The different proteins in groups and their annotations showed that adiponectin was down-regulated in obesity subtype of T2DM group, while STIM1 (stromal interaction molecule 1) was up-regulated. There were six protein kinases high expression in non-obesity DM patients, such as Serine-protein kinase ATM, Serine/threonine-protein kinase WNK1. CONCLUSION: Adiponectin, STIM1 and protein kinases may act in different roles on the pathogenesis of obesity subtype and non-obesity subtype of T2DM.