Dynamic localization of G-actin during membrane protrusion in neuronal motility.
Curr Biol
; 23(12): 1046-56, 2013 Jun 17.
Article
in En
| MEDLINE
| ID: mdl-23746641
ABSTRACT
BACKGROUND:
Actin-based cell motility is fundamental for development, function, and malignant events in eukaryotic organisms. During neural development, axonal growth cones depend on rapid assembly and disassembly of actin filaments (F-actin) for their guided extension to specific targets for wiring. Monomeric globular actin (G-actin) is the building block for F-actin but is not considered to play a direct role in spatiotemporal control of actin dynamics in cell motility.RESULTS:
Here we report that a pool of G-actin dynamically localizes to the leading edge of growth cones and neuroblastoma cells to spatially elevate the G-/F-actin ratio that drives membrane protrusion and cell movement. Loss of G-actin localization leads to the cessation and retraction of membrane protrusions. Moreover, G-actin localization occurs asymmetrically in growth cones during attractive turning. Finally, we identify the actin monomer-binding proteins profilin and thymosin ß4 as key molecules that localize actin monomers to the leading edge of lamellipodia for their motility.CONCLUSIONS:
Our results suggest that dynamic localization of G-actin provides a novel mechanism to regulate the spatiotemporal actin dynamics underlying membrane protrusion in cell locomotion and growth cone chemotaxis.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pseudopodia
/
Thymosin
/
Cell Membrane
/
Chemotaxis
/
Actins
/
Growth Cones
/
Neurons
Limits:
Animals
Language:
En
Journal:
Curr Biol
Journal subject:
BIOLOGIA
Year:
2013
Document type:
Article
Affiliation country:
Estados Unidos