A highly sensitive G-quadruplex-based luminescent switch-on probe for the detection of polymerase 3'-5' proofreading activity.
Methods
; 64(3): 224-8, 2013 Dec 15.
Article
in En
| MEDLINE
| ID: mdl-23748144
We report herein a luminescent switch-on label-free G-quadruplex-based assay for the rapid and sensitive detection of polymerase proofreading activity using a novel iridium(III) complex as a G-quadruplex-selective probe. The interaction of the iridium(III) complex with the G-quadruplex motif facilitates the highly sensitive switch-on detection of polymerase proofreading activity. Using T4 DNA polymerase (T4 pol) as a model enzyme, the assay achieved high sensitivity and selectivity for T4 pol over other tested enzymes.
Key words
ACN; Exo I; Exo III; G-quadruplex; Iridium(III) complex; Luminescent; PCR; Polymerase; Proofreading; T4 DNA polymerase; T4 pol; T7 Exo; T7 exonuclease; Taq DNA polymerase; Taq pol; acetonitrile; calf-thymus DNA; ctDNA; dAMP; dGMP; dNTP; deoxyadenosine monophosphate; deoxyguanosine monophosphate; deoxyribonucleoside triphosphate; double-stranded DNA; dsDNA; exonuclease I; exonuclease III; polymerase chain reaction; single-stranded DNA; ssDNA
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Luminescent Agents
/
DNA-Directed DNA Polymerase
/
Coordination Complexes
/
Enzyme Assays
Type of study:
Diagnostic_studies
Language:
En
Journal:
Methods
Journal subject:
BIOQUIMICA
Year:
2013
Document type:
Article
Affiliation country:
China
Country of publication:
Estados Unidos