Assessment of pathogenic bacteria using periodic actuation.
Lab Chip
; 13(16): 3192-8, 2013 Aug 21.
Article
in En
| MEDLINE
| ID: mdl-23807196
ABSTRACT
A new analytical platform for the assessment of pathogenic bacteria is presented. It is based on a robust technology which is able to amplify the signal to noise ratio providing fast and sensitive detection of target pathogenic bacteria. The system uses a custom made AC electrical impedance analyser to measure, using a lab on a chip platform, the oscillations of magnetically labelled analytes when applying a periodic magnetic field. The concentration of pathogenic Escherichia coli O157H7 chosen as bacterial model was determined based on the amplitude of the electrical impedance oscillations at a selected AC frequency. The analytical platform provides a limit of detection of 10(2) cells ml(-1), has a fast analysis time, and is amenable for the detection of other target cells. The system has simple design suitable for portability and automated operation.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Escherichia coli O157
/
Lab-On-A-Chip Devices
Type of study:
Prognostic_studies
Language:
En
Journal:
Lab Chip
Journal subject:
BIOTECNOLOGIA
/
QUIMICA
Year:
2013
Document type:
Article
Affiliation country:
Rumanía