Bone marrow stem cells in facial nerve regeneration from isolated stumps.
Muscle Nerve
; 48(3): 423-9, 2013 Sep.
Article
in En
| MEDLINE
| ID: mdl-23824709
ABSTRACT
INTRODUCTION:
Severe lesions in the facial nerve may have extensive axonal loss and leave isolated stumps that impose technical difficulties for nerve grafting.METHODS:
We evaluated bone marrow stem cells (BMSC) in a silicone conduit for rat facial nerve regeneration from isolated stumps. Group A utilized empty silicone tubes; in groups B-D, the tube was filled with acellular gel; and, in groups C and D, undifferentiated BMSC (uBMSC) or Schwann-like cells differentiated from BMSC (dBMSC) were added, respectively. Compound muscle action potentials (CMAPs) were measured, and histology was evaluated.RESULTS:
Groups C and D had the highest CMAP amplitudes. Group C had shorter CMAP durations than groups A, B, and D. Distal axonal number and density were increased in group C compared with groups A and B.CONCLUSIONS:
Regeneration of the facial nerve was improved by both uBMSC and dBMSC in rats, yet uBMSC was associated with superior functional results.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bone Marrow Transplantation
/
Muscle, Skeletal
/
Facial Nerve
/
Mesenchymal Stem Cells
/
Amputation Stumps
/
Nerve Regeneration
Type of study:
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Animals
Language:
En
Journal:
Muscle Nerve
Year:
2013
Document type:
Article
Affiliation country:
Brasil