Detection of cytomegalovirus DNA on dried blood spots collected from infants infected with HIV: an in-house method adaptable in resource-limited settings.
J Virol Methods
; 193(2): 503-7, 2013 Nov.
Article
in En
| MEDLINE
| ID: mdl-23891874
ABSTRACT
In countries with limited resources, infants infected with HIV are highly exposed to CMV co-infection which probably represents a major risk factor for disease progression in this population. This study aimed to evaluate the performance of a low cost CMV DNA extraction method from DBS and the feasibility of its implementation in laboratories of 4 countries with limited resources. DNA was extracted from DBS with a cationic resin (chelex 100) and amplified with an "in house" real time CMV PCR. Dilutions of a quantified whole blood sample were spotted on paper to evaluate the 95% detection limit. A DBS quality control panel was analyzed in all laboratories. CMV PCR was compared between DBS and liquid whole blood (gold standard) in 2 populations 418 transplanted patients and 59 infants infected with HIV (median age of 2 months). The CMV PCR 95% detection limit in DBS was 3.87 log10 copies/mL. Its positive and negative predictive values for CMV diagnosis in infants infected with HIV were 100% and 87.5% respectively. Quality control panels gave consistent qualitative results in all laboratories. This assay had high predictive values for CMV diagnosis in infants infected with HIV and its implementation in resource-limited countries with limited resources is feasible.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Specimen Handling
/
Blood
/
DNA, Viral
/
HIV Infections
/
Cytomegalovirus Infections
/
Cytomegalovirus
/
Desiccation
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
/
Qualitative_research
/
Risk_factors_studies
Limits:
Humans
/
Infant
Language:
En
Journal:
J Virol Methods
Year:
2013
Document type:
Article