Detection of salt bridges to lysines in solution in barnase.
Chem Commun (Camb)
; 49(84): 9824-6, 2013 Oct 28.
Article
in En
| MEDLINE
| ID: mdl-24030197
ABSTRACT
We show that salt bridges involving lysines can be detected by deuterium isotope effects on NMR chemical shifts of the sidechain amine. Lys27 in the ribonuclease barnase is salt bridged, and mutation of Arg69 to Lys retains a partially buried salt bridge. The salt bridges are functionally important.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ribonucleases
/
Salts
/
Lysine
Type of study:
Diagnostic_studies
/
Prognostic_studies
Language:
En
Journal:
Chem Commun (Camb)
Journal subject:
QUIMICA
Year:
2013
Document type:
Article
Affiliation country:
Reino Unido