The effect of syndecan-4 and glypican-1 expression on age-related changes in myogenic satellite cell proliferation, differentiation, and fibroblast growth factor 2 responsiveness.
Comp Biochem Physiol A Mol Integr Physiol
; 166(4): 590-602, 2013 Dec.
Article
in En
| MEDLINE
| ID: mdl-24036479
ABSTRACT
Satellite cells are multipotential stem cells responsible for muscle growth and regeneration. Satellite cell proliferation, differentiation, and responsiveness to fibroblast growth factor 2 (FGF2) is, in part, regulated by the heparan sulfate proteoglycans syndecan-4 and glypican-1. Syndecan-4 and glypican-1 expression declines with satellite cell age and may be associated with decreased satellite cell activity. The objective of the current study was to determine if overexpression of syndecan-4 and glypican-1 would increase proliferation, differentiation and FGF2 responsiveness in satellite cells isolated from pectoralis major muscle from 16-wk-old turkeys. Overexpression of syndecan-4 and glypican-1 did not have a significant effect on proliferation and differentiation in 1d, 7 wk, and 16 wk satellite cells, and did not affect FGF2 responsiveness during proliferation. Expression of syndecan-4 and glypican-1 increased differentiation at 48 h in 1d, 7 wk, and 16 wk cells treated with FGF2. Expression of myogenic regulatory factors MyoD, myogenin, and MRF4 was affected by the overexpression of syndecan-4 and glypican-1. However, changes in myogenic regulatory factor expression did not have a significant effect on proliferation or differentiation. These data demonstrate that syndecan-4 and glypican-1 are likely not directly associated with the age related decrease in satellite cell activity.
Key words
1d, 7wk, and 16wk cells; 4',6-diamidino-2-phenylindole; Age; BSA; CON; DAPI; DMEM; Dulbecco's Modified Eagle's Medium; FGF2; Fibroblast growth factor 2; G1; GAPDH; GPI; Glypican-1; MMLV; MRF4; MyoD; Myogenin; OD; PBS; PCMS-EGFP empty vector; PE; PIP(2); PKCα; PT; Paired box transcription factor 7; Pax7; RBC2; RT; RT-qPCR; Randombred Control Line 2 turkey; Ras homologue gene family, member A; RhoA; S4; S4G1; Satellite cells; Syndecan-4; bovine serum albumin; fibroblast growth factor 2; glyceraldehyde-3-phosphate dehydrogenase; glycosylphosphatidylinositol; glypican-1; moloney murine leukemia virus; myogenic differentiation 1; myogenic regulatory factor 4; optical density; phosphate buffered saline; phosphatidylinositol 4,5-bisphosphate; phycoerythrin; post-transfection; protein kinase C alpha; real-time quantitative polymerase chain reaction; room temperature; satellite cells isolated from turkeys at 1d, 7wk, and 16wk of age, respectively; syndecan-4; syndecan-4 and glypican-1
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Fibroblast Growth Factor 2
/
Satellite Cells, Skeletal Muscle
/
Glypicans
/
Syndecan-4
Limits:
Animals
Language:
En
Journal:
Comp Biochem Physiol A Mol Integr Physiol
Journal subject:
BIOLOGIA MOLECULAR
/
FISIOLOGIA
Year:
2013
Document type:
Article
Affiliation country:
Estados Unidos