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Microarray analysis of hepatic gene expression in juvenile Japanese flounder Paralichthys olivaceus fed diets supplemented with fish or vegetable oils.
Limtipsuntorn, Ubonrat; Haga, Yutaka; Kondo, Hidehiro; Hirono, Ikuo; Satoh, Shuichi.
Affiliation
  • Limtipsuntorn U; Department of Marine Bioscience, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan, Minato 4-5-7, Tokyo, 108-8477, Japan.
Mar Biotechnol (NY) ; 16(1): 88-102, 2014 Feb.
Article in En | MEDLINE | ID: mdl-24052493
Gene expression profiling was performed in Japanese flounder Paralichthys olivaceus fed diets supplemented with fish oil (FO), linseed oil (LO), or olive oil (OO) for 6 weeks. The LO and OO groups showed significantly retarded growth, lower feed intake, lower protein efficiency ratio, and lower hepatosomatic index (P < 0.05). Liver fatty acid composition reflected the dietary fatty acid composition. Microarray analysis revealed that dietary n - 3 highly unsaturated fatty acid (HUFA) deficiency affected 169 transcripts. In the LO group, 57 genes were up-regulated and 38 genes were down-regulated, whereas in the OO group nine genes were up-regulated and 87 genes were down-regulated. Analysis of the functional annotations suggested that dietary n - 3 HUFA affected genes involved in signal transduction (23.2 %), cellular processes (21.1 %), metabolism (including glucose, lipid, and nucleobase; 15.5 %), transport (11.3 %), regulation of transcription (10.5 %), and immune response (4.2 %). Several genes encoding serine/threonine kinases such as protein kinase C and calmodulin-dependent kinase and nuclear hormone receptors such as vitamin D receptor, retinoic acid receptor, and receptors for cytokines (bone morphogenic protein and transforming growth factor ß) were affected. Among 169 transcripts, 22 genes were affected in both LO and OO groups. The present study identified several genes involved in n - 3 HUFA deficiency-sensitive pathways, which will be useful for selective breeding of flounder strains able to adapt to n - 3 HUFA deficiency.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Flounder / Plant Oils / Fish Oils / Gene Expression Regulation / Dietary Supplements / Liver Type of study: Prognostic_studies Limits: Animals Language: En Journal: Mar Biotechnol (NY) Journal subject: BIOLOGIA / BIOTECNOLOGIA Year: 2014 Document type: Article Affiliation country: Japón Country of publication: Estados Unidos

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Flounder / Plant Oils / Fish Oils / Gene Expression Regulation / Dietary Supplements / Liver Type of study: Prognostic_studies Limits: Animals Language: En Journal: Mar Biotechnol (NY) Journal subject: BIOLOGIA / BIOTECNOLOGIA Year: 2014 Document type: Article Affiliation country: Japón Country of publication: Estados Unidos