Pretreatment of urine samples with SDS improves direct identification of urinary tract pathogens with matrix-assisted laser desorption ionization-time of flight mass spectrometry.
J Clin Microbiol
; 52(1): 335-8, 2014 Jan.
Article
in En
| MEDLINE
| ID: mdl-24226916
ABSTRACT
We pretreated with SDS 71 urine samples with bacterial counts of >10(5) CFU/ml and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) identification scores of <2, in order to minimize failure rates. Identification improved in 46.5% of samples, remained unchanged in 49.3%, and worsened in 4.2%. The improvement was more evident for Gram-negative (54.3%) than for Gram-positive (32%) bacteria.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Specimen Handling
/
Bacteria
/
Bacterial Infections
/
Urinary Tract Infections
/
Urine
/
Microbiological Techniques
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
J Clin Microbiol
Year:
2014
Document type:
Article
Affiliation country:
España