Influence of the excision shock on the protein metabolism ofVicia faba L. meristematic root cells.
Planta
; 155(6): 478-85, 1982 Nov.
Article
in En
| MEDLINE
| ID: mdl-24272113
UsingVicia faba root meristems we have shown that protein synthesis was dramatically changed after excision. The amino-acid incorporation dropped to 13% of the level in the unexcised control. This downshift was a direct consequence of the breakdown of polysomes which are converted into monosomes. In order to perform an analysis of the protein pattern by two-dimensional gel electrophoresis, endogenous proteolytic activity, which is high in broad bean root, had to be inhibited. Therefore, several protease inhibitors were tested and a very efficient inhibitor pool was obtained which could be used during the preparation of meristematic cell extracts. Protein-pattern analysis showed important differences between the unexcised control and excised apices. The number of proteins synthesized after excision droped from 250 in the control to 80, as a consequence of polysome breakdown. Futhermore, we present evidence that new and apparently specific proteins are synthesized in response to this excision shock.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Planta
Year:
1982
Document type:
Article
Affiliation country:
Francia
Country of publication:
Alemania