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Light-activated serotonin for exploring its action in biological systems.
Rea, Adam C; Vandenberg, Laura N; Ball, Rebecca E; Snouffer, Ashley A; Hudson, Alicia G; Zhu, Yue; McLain, Duncan E; Johnston, Lindsey L; Lauderdale, James D; Levin, Michael; Dore, Timothy M.
Affiliation
  • Rea AC; Department of Chemistry, University of Georgia, Athens, GA 30602, USA.
  • Vandenberg LN; Biology Department and Tufts Center for Regenerative and Developmental Biology, Tufts University, Suite 4600, 200 Boston Avenue, Medford, MA 02155-4243, USA.
  • Ball RE; Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA.
  • Snouffer AA; Department of Genetics, University of Georgia, Athens, GA 30602, USA.
  • Hudson AG; Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA.
  • Zhu Y; Department of Chemistry, University of Georgia, Athens, GA 30602, USA.
  • McLain DE; Department of Chemistry, University of Georgia, Athens, GA 30602, USA; New York University Abu Dhabi, P.O. Box 129188, Abu Dhabi, United Arab Emirates.
  • Johnston LL; Department of Genetics, University of Georgia, Athens, GA 30602, USA.
  • Lauderdale JD; Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA.
  • Levin M; Biology Department and Tufts Center for Regenerative and Developmental Biology, Tufts University, Suite 4600, 200 Boston Avenue, Medford, MA 02155-4243, USA.
  • Dore TM; Department of Chemistry, University of Georgia, Athens, GA 30602, USA; New York University Abu Dhabi, P.O. Box 129188, Abu Dhabi, United Arab Emirates. Electronic address: timothy.dore@nyu.edu.
Chem Biol ; 20(12): 1536-46, 2013 Dec 19.
Article in En | MEDLINE | ID: mdl-24333002
ABSTRACT
Serotonin (5-HT) is a neuromodulator involved in regulating mood, appetite, memory, learning, pain, and establishment of left-right (LR) asymmetry in embryonic development. To explore the role of 5-HT in physiology, we have created two forms of "caged" 5-HT, BHQ-O-5HT and BHQ-N-5HT. When exposed to 365 or 740 nm light, BHQ-O-5HT releases 5-HT through one- or two-photon excitation, respectively. BHQ-O-5HT mediated changes in neural activity in cultured mouse primary sensory neurons and the trigeminal ganglion and optic tectum of intact zebrafish larvae in the form of high-amplitude spiking in response to light. In Xenopus laevis embryos, light-activated 5-HT increased the occurrence of LR patterning defects. Maximal rates of LR defects were observed when 5-HT was released at stage 5 compared with stage 8. These experiments show the potential for BHQ-caged serotonins in studying 5-HT-regulated physiological processes.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Serotonin / Light Limits: Animals Language: En Journal: Chem Biol Journal subject: BIOLOGIA / BIOQUIMICA / QUIMICA Year: 2013 Document type: Article Affiliation country: Estados Unidos
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Serotonin / Light Limits: Animals Language: En Journal: Chem Biol Journal subject: BIOLOGIA / BIOQUIMICA / QUIMICA Year: 2013 Document type: Article Affiliation country: Estados Unidos