Association analysis of bovine Foxa2 gene single sequence variant and haplotype combinations with growth traits in Chinese cattle.
Gene
; 536(2): 385-92, 2014 Feb 25.
Article
in En
| MEDLINE
| ID: mdl-24333857
ABSTRACT
Forkhead box A2 (Foxa2) has been recognized as one of the most potent transcriptional activators that is implicated in the control of feeding behavior and energy homeostasis. However, similar researches about the effects of genetic variations of Foxa2 gene on growth traits are lacking. Therefore, this study detected Foxa2 gene polymorphisms by DNA pool sequencing, PCR-RFLP and PCR-ACRS methods in 822 individuals from three Chinese cattle breeds. The results showed that four sequence variants (SVs) were screened, including two mutations (SV1, g. 7005 C>T and SV2, g. 7044 C>G) in intron 4, one mutation (SV3, g. 8449 A>G) in exon 5 and one mutation (SV4, g. 8537 T>C) in the 3'UTR. Notably, association analysis of the single mutations with growth traits in total individuals (at 24months) revealed that significant statistical difference was found in four SVs, and SV4 locus was highly significantly associated with growth traits throughout all three breeds (P<0.05 or P<0.01). Meanwhile, haplotype combination CCCCAGTC also indicated remarkably associated to better chest girth and body weight in Jiaxian Red cattle (P<0.05). We herein described a comprehensive study on the variability of bovine Foxa2 gene that was predictive of molecular markers in cattle breeding for the first time.
Key words
ANOVA; Analysis of variance; Association; Cattle; DLK1; Delta-like 1; EM; Expectation maximization; FTO; Fat mass and obesity associated; Forkhead box A2; Foxa2; Foxa2 gene; G6PC2; Growth traits; HNF-3; HWE; Haplotype combination; HardyWeinberg equilibrium; Hepatocyte nuclear factor 3; JX; Jiaxian Red; LD; Linkage disequilibrium; NY; Nanyang; PCR-ACRS; PCR-based amplification-created restriction site; PCRRFLP; PIC; Polymerase chain reactionrestriction fragment length polymorphism; Polymorphism information content; QC; Qinchuan; SNPs; SVs; Sequence variant; Sequence variants; Single nucleotide polymorphisms; UTR; Unstranslated region(s); glucose-6-phosphatase catalytic subunit 2
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Haplotypes
/
Polymorphism, Single Nucleotide
/
Hepatocyte Nuclear Factor 3-beta
Type of study:
Prognostic_studies
/
Risk_factors_studies
Limits:
Animals
Language:
En
Journal:
Gene
Year:
2014
Document type:
Article
Affiliation country:
China