In vitro vascularization of a combined system based on a 3D printing technique.
J Tissue Eng Regen Med
; 10(10): 833-842, 2016 10.
Article
in En
| MEDLINE
| ID: mdl-24399638
ABSTRACT
A vital challenge in complex organ manufacturing is to vascularize large combined tissues. The aim of this study is to vascularize in vitro an adipose-derived stem cell (ADSC)/fibrin/collagen incorporated three-dimensional (3D) poly(d,l-lactic-co-glycolic acid) (PLGA) scaffold (10 × 10 × 10 mm3 ) with interconnected channels. A low-temperature 3D printing technique was employed to build the PLGA scaffold. A step-by-step cocktail procedure was designed to engage or steer the ADSCs in the PLGA channels towards both endothelial and smooth muscle cell lineages. The combined system had sufficient mechanical properties to support the cell/fibrin/collagen hydrogel inside the predefined PLGA channels. The ADSCs encapsulated in the fibrin/collagen hydrogel differentiated to endothelial and smooth muscle cell lineage, respectively, corresponding to their respective locations in the construct and formed vascular-like structures. This technique allows in vitro vascularization of the predefined PLGA channels and provides a choice for complex organ manufacture. Copyright © 2014 John Wiley & Sons, Ltd.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polyglycolic Acid
/
Stem Cells
/
Fibrin
/
Adipose Tissue
/
Collagen
/
Neovascularization, Physiologic
/
Lactic Acid
/
Tissue Scaffolds
Limits:
Animals
Language:
En
Journal:
J Tissue Eng Regen Med
Journal subject:
BIOTECNOLOGIA
/
HISTOLOGIA
Year:
2016
Document type:
Article
Affiliation country:
China