Inhibitory effects of PLAP-1/asporin on periodontal ligament cells.
J Dent Res
; 93(4): 400-5, 2014 Apr.
Article
in En
| MEDLINE
| ID: mdl-24453179
ABSTRACT
PLAP-1/asporin is an extracellular matrix protein that is predominantly expressed in the human periodontal ligament (PDL) and has an aspartic acid (D) repeat polymorphism in its N-terminal region. In this study, we hypothesized that the D repeat polymorphism of PLAP-1/asporin may affect the physiological functions of periodontal ligaments. We established periodontal ligament cell lines transfected with the D13- or D14-PLAP-1 gene. Alkaline phosphatase staining and alizarin red staining revealed that the cytodifferentiation of the D14-PLAP-1-expressing PDL cells was more repressed compared with that of the D13-PLAP-1-expressing cells. Furthermore, the D14-PLAP-1-expressing cells inhibited BMP-2-induced cytodifferentiation more strongly than did the D13-PLAP-1-expressing cells. Western blotting analysis and luciferase assay revealed that D14-PLAP-1 suppressed BMP-2 signal transduction more efficiently than did D13-PLAP-1, and co-immunoprecipitation demonstrated the stronger affinity of the D14-PLAP-1 protein to BMP-2 compared with the D13-PLAP-1 protein. Analysis of these data suggests that the D repeat polymorphism of PLAP-1/asporin has a significant influence on the functions of PDL cells.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Periodontal Ligament
/
Extracellular Matrix Proteins
Limits:
Humans
Language:
En
Journal:
J Dent Res
Year:
2014
Document type:
Article
Affiliation country:
Japón