A cell-free approach to accelerate the study of protein-protein interactions in vitro.
Interface Focus
; 3(5): 20130018, 2013 Oct 06.
Article
in En
| MEDLINE
| ID: mdl-24511386
ABSTRACT
Protein-protein interactions are highly desirable targets in drug discovery, yet only a fraction of drugs act as binding inhibitors. Here, we review the different technologies used to find and validate protein-protein interactions. We then discuss how the novel combination of cell-free protein expression, AlphaScreen and single-molecule fluorescence spectroscopy can be used to rapidly map protein interaction networks, determine the architecture of protein complexes, and find new targets for drug discovery.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Interface Focus
Year:
2013
Document type:
Article
Affiliation country:
Australia