Diversification of 13S globulins, allergenic seed storage proteins, of common buckwheat.
Food Chem
; 155: 192-8, 2014 Jul 15.
Article
in En
| MEDLINE
| ID: mdl-24594174
ABSTRACT
The α polypeptide of the 13S globulin subunit of common buckwheat is the counterpart of the major allergenic ß polypeptide. Trypsin digestibility varies between variants of the α polypeptide with and without a tandem repeat insert. To evaluate the intra-species diversity of 13S globulin, the comprehensive screening of a genomic DNA library was performed, resulting in the isolation of 14 and 3 genes for Met-poor and Met-rich subunits, respectively. Although most tandem repeat units were 45 bp in length, the two-repeat gene Glb2B and all one-repeat genes contained an additional 3 bp. Secondary structure predictions and polyacrylamide gel electrophoresis demonstrated that the sense strand of Glb2B-CCG, the additional 3 bp-deletion clone of Glb2B, formed a more rigid secondary structure than that of the wild-type. Thus, the large intra-species variation of 13S globulin revealed in this study and its diversification might be attributable to the unique nature of the tandem repeat sequences.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Allergens
/
Fagopyrum
/
Seed Storage Proteins
/
Globulins
Language:
En
Journal:
Food Chem
Year:
2014
Document type:
Article
Affiliation country:
Japón