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Evolutionary conservation of TFIIH subunits: implications for the use of zebrafish as a model to study TFIIH function and regulation.
Silva, I A L; Cox, C J; Leite, R B; Cancela, M L; Conceição, N.
Affiliation
  • Silva IA; Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal; Centre of Marine Sciences (CCMAR), University of Algarve, Faro, Portugal.
  • Cox CJ; Centre of Marine Sciences (CCMAR), University of Algarve, Faro, Portugal.
  • Leite RB; Centre of Marine Sciences (CCMAR), University of Algarve, Faro, Portugal.
  • Cancela ML; Department of Biomedical Sciences and Medicine, University of Algarve, Faro, Portugal; Centre of Marine Sciences (CCMAR), University of Algarve, Faro, Portugal.
  • Conceição N; Centre of Marine Sciences (CCMAR), University of Algarve, Faro, Portugal. Electronic address: nconcei@ualg.pt.
Article in En | MEDLINE | ID: mdl-24731924
Transcriptional factor IIH (TFIIH) is involved in cell cycle regulation, nucleotide excision repair, and gene transcription. Mutations in three of its subunits, XPB, XPD, and TTDA, lead to human recessive genetic disorders such as trichothiodystrophy and xeroderma pigmentosum, the latter of which is sometimes associated with Cockayne's syndrome. In the present study, we investigate the sequence conservation of TFIIH subunits among several teleost fish species and compare their characteristics and putative regulation by transcription factors to those of human and zebrafish. We report the following findings: (i) comparisons among protein sequences revealed a high sequence identity for each TFIIH subunit analysed; (ii) among transcription factors identified as putative regulators, OCT1 and AP1 have the highest binding-site frequencies in the promoters of TFIIH genes, and (iii) TFIIH genes have alternatively spliced isoforms. Finally, we compared the protein primary structure in human and zebrafish of XPD and XPB - two important ATP-dependent helicases that catalyse the unwinding of the DNA duplex at promoters during transcription - highlighting the conservation of domain regions such as the helicase domains. Our study suggests that zebrafish, a widely used model for many human diseases, could also act as an important model to study the function of TFIIH complex in repair and transcription regulation in humans.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Evolution, Molecular / Transcription Factor TFIIH Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Comp Biochem Physiol B Biochem Mol Biol Journal subject: BIOLOGIA MOLECULAR / BIOQUIMICA Year: 2014 Document type: Article Affiliation country: Portugal Country of publication: Reino Unido

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Evolution, Molecular / Transcription Factor TFIIH Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Comp Biochem Physiol B Biochem Mol Biol Journal subject: BIOLOGIA MOLECULAR / BIOQUIMICA Year: 2014 Document type: Article Affiliation country: Portugal Country of publication: Reino Unido