Function of mechanically lengthened jejunum after restoration into continuity.
J Pediatr Surg
; 49(6): 971-4; discussion 974-5, 2014 Jun.
Article
in En
| MEDLINE
| ID: mdl-24888845
ABSTRACT
PURPOSE:
Distraction enterogenesis is a potential treatment for patients with short bowel syndrome. We previously demonstrated successful lengthening of jejunum using a degradable spring device in rats. Absorptive function of the lengthened jejunum after restoration into intestinal continuity needs to be determined.METHODS:
Encapsulated polycaprolactone springs were placed into isolated jejunal segments in rats for four weeks. Lengthened segments of jejunum were subsequently restored into intestinal continuity. Absorption studies were performed by placing a mixture of a non-absorbable substrate and glucose into the lumen of the restored jejunum.RESULTS:
Restored jejunal segments demonstrated visible peristalsis at specimen retrieval. Compared to normal jejunal controls, restored segments demonstrated equal water absorption and greater glucose absorption. Restored segments had thicker smooth muscle, increased villus height, increased crypt depth, and decreased sucrase activity compared to normal jejunum. The density of enteric ganglia increased after restoration to near normal levels in the submucosa and to normal levels in the myenteric plexus.CONCLUSION:
Jejunum lengthened with a degradable device demonstrates peristaltic and enzymatic activity as well as glucose and water absorption after restoration into intestinal continuity. Our findings further demonstrate the therapeutic potential of a degradable device.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peristalsis
/
Short Bowel Syndrome
/
Tissue Expansion
/
Jejunum
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J Pediatr Surg
Year:
2014
Document type:
Article
Affiliation country:
Estados Unidos
Publication country:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA