Dynamic contrast-enhanced ultrasound of slaughterhouse porcine livers in machine perfusion.
Ultrasound Med Biol
; 40(9): 2217-30, 2014 Sep.
Article
in En
| MEDLINE
| ID: mdl-25023101
ABSTRACT
The aim of this study was to enable investigations into novel imaging and surgical techniques by developing a readily accessible, versatile liver machine perfusion system. Slaughterhouse pig livers were used, and dynamic contrast-enhanced ultrasound was introduced to optimize the procurement process and provide real-time perfusion monitoring. The system comprised a single pump, oxygenator, bubble trap and two flowmeters for pressure-controlled perfusion of the vessels using an off-the-shelf perfusate at room temperature. Successful livers exhibited homogeneous perfusion in both the portal vein and hepatic artery with dynamic contrast-enhanced ultrasound, which correlated with stable oxygen uptake, bile production and hepatic resistance and normal histology at the end of 3 h of perfusion. Dynamic contrast-enhanced ultrasound revealed perfusion abnormalities invisible to the naked eye, thereby providing context to the otherwise systemic biochemical/hemodynamic measurements and focal biopsy findings. The model developed here is a simple, cost-effective approach for stable ex vivo whole-organ machine perfusion.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Perfusion
/
Image Enhancement
/
Contrast Media
/
Liver
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Ultrasound Med Biol
Year:
2014
Document type:
Article
Affiliation country:
Chipre