A next-generation dual-recombinase system for time- and host-specific targeting of pancreatic cancer.
Nat Med
; 20(11): 1340-1347, 2014 Nov.
Article
in En
| MEDLINE
| ID: mdl-25326799
ABSTRACT
Genetically engineered mouse models (GEMMs) have dramatically improved our understanding of tumor evolution and therapeutic resistance. However, sequential genetic manipulation of gene expression and targeting of the host is almost impossible using conventional Cre-loxP-based models. We have developed an inducible dual-recombinase system by combining flippase-FRT (Flp-FRT) and Cre-loxP recombination technologies to improve GEMMs of pancreatic cancer. This enables investigation of multistep carcinogenesis, genetic manipulation of tumor subpopulations (such as cancer stem cells), selective targeting of the tumor microenvironment and genetic validation of therapeutic targets in autochthonous tumors on a genome-wide scale. As a proof of concept, we performed tumor cell-autonomous and nonautonomous targeting, recapitulated hallmarks of human multistep carcinogenesis, validated genetic therapy by 3-phosphoinositide-dependent protein kinase inactivation as well as cancer cell depletion and show that mast cells in the tumor microenvironment, which had been thought to be key oncogenic players, are dispensable for tumor formation.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Genetic Engineering
/
Carcinoma, Pancreatic Ductal
/
Recombinases
/
Precision Medicine
/
Molecular Targeted Therapy
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Nat Med
Journal subject:
BIOLOGIA MOLECULAR
/
MEDICINA
Year:
2014
Document type:
Article
Affiliation country:
Alemania