In situ characterization of mycobacterial growth inhibition by lytic enzymes expressed in vectorized E. coli.
ACS Synth Biol
; 3(12): 932-4, 2014 Dec 19.
Article
in En
| MEDLINE
| ID: mdl-25408994
The emergence of extremely drug resistant Mycobacterium tuberculosis necessitates new strategies to combat the pathogen. Engineered bacteria may serve as vectors to deliver proteins to human cells, including mycobacteria-infected macrophages. In this work, we target Mycobacterium smegmatis, a nonpathogenic tuberculosis model, with E. coli modified to express trehalose dimycolate hydrolase (TDMH), a membrane-lysing serine esterase. We show that TDMH-expressing E. coli are capable of lysing mycobacteria in vitro and at low pH. Vectorized E. coli producing TDMH were found suppress the proliferation of mycobacteria in infected macrophages.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mycobacterium smegmatis
/
Escherichia coli
/
Esterases
/
Bioengineering
/
Genetic Vectors
Limits:
Humans
Language:
En
Journal:
ACS Synth Biol
Year:
2014
Document type:
Article
Affiliation country:
Francia
Country of publication:
Estados Unidos